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A developmentally regulated glycoprotein implicated in adhesion of Dictyostelium slugs in predominantly in prespore cells
Author(s) -
Künzli Marianne,
Parish Roger W.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80615-7
Subject(s) - dictyostelium discoideum , microbiology and biotechnology , glycoprotein , biology , slime mold , adhesion , dictyostelium , mycetozoa , cell adhesion , slug , cell sorting , actin , myosin , cell , biochemistry , chemistry , gene , organic chemistry , cancer research
Prestalk and prespore cells were isolated from Dictyostelium discoideum slugs after [1‐ 14 C]acetate labelling and the distribution of developmentally regulated plasma membrane proteins between the two cells types determined. A glycoprotein ( M r 95 000) involved in slug cell adhesion, and all the developmentally regulated plasma membrane proteins synthesized during the latter half of development, were predominantly associated with prespore cells. Sorting‐out patterns in slugs indicated that cell adhesion is greater between prespore than prestalk cells. The M r 95 000 glycoprotein may be part of the cell recognition and sorting mechanisms involved in fruiting body formation. Actin and myosin synthesis ceased in prespore but not prestalk cells. When slugs were disaggregated, synthesis of these two proteins was reactivated in prespore cells, but synthesis of developmentally regulated plasma membrane proteins was not induced in prestalk cells. Actin and myosin synthesis was activated in prespore cells following disaggregation.