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Photoaffinity labeling of chloroplast cytochrome b 6 ‐ f complex by an inhibitor azido‐derivative
Author(s) -
Oettmeier Walter,
Masson Klaus,
Olschewski Ellen
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80612-1
Subject(s) - plastocyanin , photoaffinity labeling , electron transport chain , chloroplast , cytochrome f , cytochrome b6f complex , thylakoid , chemistry , protein subunit , cytochrome b , cytochrome , stereochemistry , covalent bond , affinity label , biochemistry , photochemistry , photosystem i , binding site , enzyme , organic chemistry , mitochondrial dna , gene
The photoaffinity label 2‐azido‐2′,4,4′‐trinitro‐6‐ sec. butyldiphenylether inhibits photosynthetic electron transport in the thylakoid membrane as well as plastohydroquinone‐plastocyanin‐oxidoreductase activity in the isolated chloroplast cytochrome b 6 ‐ f complex. In the latter, the 14 C‐labeled inhibitor upon UV‐irradiation binds covalently only to the cytochrome b 6 and the Rieske Fe‐S peptide subunit.