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Effect of b‐itc 1 ‐site inhibitors on the midpoint potentials of mitochondrial cytochromes b
Author(s) -
Kunz Wolfram S.,
Konstantinov Alexander A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80611-x
Subject(s) - chemistry , submitochondrial particle , cytochrome b , titration , respiratory chain , potentiometric titration , antimycin a , cytochrome , stereochemistry , electron transport chain , biochemistry , mitochondrion , enzyme , inorganic chemistry , electrode , mitochondrial dna , gene
Anaerobic potentiometric titrations of b cytochromes have been carried out in beef heart submitochondrial particles in the presence of several specific inhibitors of electron transfer through the b‐c 1 ‐site of the respiratory chain. Whereas antimycin shows no significant effect on the titration curve of cytochrome b ‐562, NoHOQnO is found to shift the E m of b ‐562 by 20–30 mV to the positive. Funiculosin raises the E m of b ‐562 by > 100 mV and also appears to bring about a minor shift of b ‐566 midpoint potential. In the presence of myxothiazol, both b cytochromes with E m values 15–30 mV more positive than in the control.