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NADH oxidation in phospholipid‐enriched cytoplasmic membrane vesicles from Escherichia coli
Author(s) -
Demant Erland J.F.,
Jensen Poul K.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80601-7
Subject(s) - vesicle , phospholipid , biochemistry , escherichia coli , chemistry , oxidase test , nadh dehydrogenase , dehydrogenase , liposome , enzyme , membrane , protein subunit , gene
NADH oxidation in Escherichia coli cytoplasmic membrane vesicles enriched in anionic phospholipids by de novo synthesis of lipid in the vesicles from acyl‐CoA esters and sn ‐glycerol 3‐phosphate has been studied. NADH‐oxidase but not NADH‐dehydrogenase activity was found to decrease during synthesis and accumulation of phospholipid in the vesicles. Density gradient fractionation showed that NADH‐oxidase activity was reduced to ≈30% in vesicles with a 3–6‐fold increase in anionic phospholipid, whereas vesicles with a > 10‐fold increase in phospholipid had virtually no NADH oxidase activity.