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Complex formation of guanidinated bovine trypsin inhibitor (Kunitz) with trypsin, chymotrypsin and trypsinogen as studied by the spin‐label technique
Author(s) -
Wenzel H.R.,
Tschesche H.,
Von Goldammer E.,
Netzelmann U.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80519-x
Subject(s) - trypsin , trypsinogen , chemistry , enzyme , biochemistry
1. Introduction Studying the interaction between protein protein- ase inhibitors and their target enzymes by physico- chemical methods has often been hampered by the lack of suitable intrinsic spectroscopic signals, which change upon complex formation. specifically spin-labelled at the amino-terminus and its complexes with trypsin, chymotrypsin and trypsin- ogen were chosen as a first test system. 2. Experimental 2.1.