Premium
Increase in the number of glucose carriers in chick fibroblasts during embryo development
Author(s) -
Bernard B.,
Codogno P.,
Berjonneau C.,
Aubrey M.,
Bourrillon R.
Publication year - 1982
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(82)80189-0
Subject(s) - cytochalasin b , hexose , embryo , glucose uptake , glucose transporter , biology , chemistry , biochemistry , medicine , endocrinology , microbiology and biotechnology , cell , enzyme , insulin
Cytochalasin B (CB) has been used as a tool to ascertain whether the increase in the rate of 3‐ O ‐methylglucose (3‐O‐MeG) uptake between the 8th and the 16th day of development in chick embryo fibroblasts could be attributed to an increase in the number of hexose transport carriers. There was a 2—3‐fold difference in glucose‐specific CB binding between the 8‐ and the 16‐day cells, a difference which is comparable to the previously reported differences in rates of 3‐O‐MeG uptake. We therefore suggest that glucose‐specific CB binding represents binding to the 3‐O‐MeG carrier and that the increase in the rate of 3‐O‐MeG uptake from the 8th and the 16th day of development is probably due to an increase in the number of hexose carriers.