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Nucleodisome — a new repeat unit of chromatin revealed in nuclei of pigeon erythrocytes by DNase I digestion
Author(s) -
Khachatrian A.T.,
Pospelov V.A.,
Svetlikova S.B.,
Vorob'ev V.I.
Publication year - 1981
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(81)81087-3
Subject(s) - cytology , library science , citation , biology , genetics , computer science
It is well known that eucaryotic chromatin is arranged as linear repeat of structural units (nucleosomes) [l-3]. DNA, linking nucleosomes (linker DNA) shows an increased sensitivity to the action of different nucleases. Electrophoresis of DNA fragments isolated from nuclease-digested cl~romatin reveals a ‘ladder’ of fragments which are multiple in size to DNA length of an elementary subunit (a nucleosome). As shown here, the treatment of pigeon erythrocyte nuclei with pancreatic DNase I enabled us to detect a series of DNA fragments the length of which is multiple to the duplicate length of the nucleosome DNA repeat. This implies the presence in avian erythrocytes of a particular DNase I-resistant structure consisting of two nucleosomes (nucleodisomes).