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Primary translation product of mRNA coding for rat duodenal vitamin D‐dependent calcium‐binding protein
Author(s) -
Thomasset Monique,
Desplan Claude,
Moukhtar Moshen,
Mathieu Henri
Publication year - 1981
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(81)80596-0
Subject(s) - humanities , microbiology and biotechnology , biology , art
The intestinal mucosa of several species contains vitamin D-dependent calcium-binding proteins (CaBP) which may be involved in the complex process of calcium transport [ 11. The cytosolic CaBP (11 500 Mr) [2,3] of the rat duodenum accounts for 2% of the soluble mucosal protein [4]. It also exhibits considerable specificity since it has no immuno-crossreactivity with either other mammalian intestinal CaBPs [5], including porcine CaBP [5-71, or the larger (28 000 Mr) [8] vitamin D-dependent CaBP of the chick duodenal mucosa [ 51. The induction of intestinal 28 000 Mr CaBP synthesis by active messenger RNA (mRNA) has been studied in the chick [9-121. It appears to be dependent on the hormonal metabolite of vitamin D, 1,25-dihydroxycholecalciferol (l,25-(OH)2D3) [ 111. In the rat there is evidence indicating that the 11 500 Mr duodenal CaBP concentration measured by using radioimmunoassay is dependent upon 1,25-(OH)2Da [13,14]. To date, however, there is no report of its de novo synthesis. To further study the regulation of rat duodenal CaBP production we have undertaken an analysis of the expression of the relevant gene(s). This report deals with the identification of the primary translation product of the mRNA coding for rat duodenal CaBP.