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Influx of Ca 2+ into isolated secretory vesicles from adrenal medulla
Author(s) -
Krieger-Brauer Heidemarie,
Gratzl Manfred
Publication year - 1981
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(81)80515-7
Subject(s) - chemistry , citation , library science , computer science
Secretory vesic1es from adrenal medulla containcatecholamines, nuc1eotides and proteins, all of whichare released into the extracellular fluid during exocytosis.Adrenal medullary secretory vesic1es also containhigh concentrations of Ca'+ [1]. The mechanismof the aecumulation of Ca 2+ into the vesicles is largelyunknown and the experimental data eoncerning theuptake of Ca'+ into isolated secretory vesicles arecontradictory. It has been reported that secretoryvesicle membranes are impermeable to Ca'+ [2], thatsecretory vesicles take up Ca 2+ independently of ATP[3] and that they possess an ATP-stimulated uptakesystem [4,5].In earlier work relatively impure and unstableseeretory vesicle fractions were used for the determinationof Ca 2+ -uptake. We have developed a methodto isolate highly purified and stable secretory vesiclesfrom bovine medulla [6]. With these vesic1es werepeated earlier Ca'+ -uptake experiments and foundthat:(i) The vesic1es take up