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Interaction of chromaffin granules with plasma membranes mediated by Ca 2+ and Mg 2+ ‐ATP using self‐generating gradients of percoll®
Author(s) -
Grafenstein Hermannvon,
Neumann Eberhard
Publication year - 1981
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(81)80296-7
Subject(s) - percoll , physics , chemistry , stereochemistry , philosophy , biochemistry , centrifugation
The chromaffin granules (vesicles storing catecholamines, nucleotides and other components of adrenal medullary cells [I] represent a classic model system for exocytotic hormone release from secretory cells [2]. Exocytosis is assumed to involve binding of the storage vesicles to and fusion with, the plasma membrane, and possibly dissociation of (empty) vesicles from exocytotic sites. Recent evidence suggests that in chromaffin cells at least two components are essential and specific for exocytotic release: Ca2’ and Mg-ATP [ 31. Here, chromaffin cell homogenates are used to investigate the effect of Ca2+ and Mg-ATP on the interaction of granules with fragments of plasma membranes. Although homogenization of tissue certainly destroys cytoplasmatic structures, essential components of the release system remain present. The main results of this in vitro study [4] are: (1)