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Interaction of cytoplasmic proteins with liposomes and their cell specificity
Author(s) -
Utsumi Kozo,
Okimasu Eiji,
Takehara Yoshiki,
Watanabe Sadahiro,
Miyara Masanobu,
Moromizato Yasuki
Publication year - 1981
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(81)80150-0
Subject(s) - medical school , medicine , medical education
Numerous studies have shown that the plasma membrane has a fundamental role in the regulation of cell metabolism [l-4]. Attention has been focussed on the transmembrane control mechanism concerning the interaction between plasma membrane and cytoskeletal structures [2-121. For the transfer of biological information from external surface to cytoplasm, 3 steps are postulated: (i) Interaction of surface receptors with ligands at the external cell surface; (ii) Transmission of the information across the plasma membrane; (iii) Modulation of cytoplasmic components at the inner surface of the membrane. For example, cell agglutination by concanavalin A (con A) is accompanied by the redistribution of con A receptors on the cell surface from a random pattern to form a cap [2], and microtubules and microfilaments are involved in the transmembrane control of redistribution of lectin receptor sites in the cell membrane [2,3]. Therefore, the interaction of cytoplasmic proteins with the plasma membrane through their lipophilic nature and affinity to the membrane-integrated proteins have an important role for the regulatory mechanism of the transmembrane control system in cells. Here we report our attempts to determine the existence of an interaction between cytoplasmic proteins and artificial lipid vesicles, liposomes. We find that cytoskeletal components such as tubulin, actin and ol-actinin have a strong tendency to associate with liposomes and that this characteristic, which may be altered by several physiological conditions, is respon-