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Horse spleen ferritin: a change of crystal form during crystallisation of monomer is associated with the appearance of oligomers
Author(s) -
Saeed S.A.,
Boyde T.R.C.
Publication year - 1981
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(81)80031-2
Subject(s) - newcastle upon tyne , newcastle disease , new england , medicine , library science , politics , history , political science , law , art history , virology , computer science , virus
The conventional preparation of ferritin from horse spleen includes crystallisation in the presence of cadmium sulphate. The product is heterogeneous to electrophoresis on starch or polyacrylamide gel because some of the spherical units exist as dimers, trimers and higher oligomers [l-3]. The nature and origin of ferritin oligomers is not known. We have now separated the ferritin monomer and the individual polymeric forms by preparative polyacrylamide gel electrophoresis and have found that formation of oligomers occurs during the crystallisation of ferritin monomer.