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Na + transport via Na + /H + antiport in Halobacterium halobium envelope vesicles
Author(s) -
Luisi Bonaventura F.,
Lanyi Janos K.,
Weber H.Jürgen
Publication year - 1980
Publication title -
febs letters
Language(s) - Slovenian
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(80)80979-3
Subject(s) - national laboratory , physics , chemistry , nanotechnology , engineering physics , materials science
Using H. halobium cell envelope vesicles containing either bacteriorhodopsin plus Na+ pump, bacteriorhodopsin alone, Na+ pump alone, or no light-responsive pigment altogether, it could be shown that the large majority of light energized Na+ extrusion in these mutants is linked to bacteriorhodopsin and to protonmotive force, and therefore must be facilitated by a Na+/H+ antiporter. Thus, the recently discovered primary Na+ pump makes only a minor contribution to light-mediated Na+ flux. The activity of the Na+/H+ antiporter appears to be independent of the presence of any photoreactive pigments, since an artifical electron donor will drive rapid Na+ extrusion in all of the vesicle preparations tested.