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On the mechanism of single‐stranded RNA synthesis by encephalomyocarditis virus replication complexes
Author(s) -
Dmitrieva T.M.,
Eremeeva T.P.,
Alatortseva G.I.,
Agol V.I.
Publication year - 1980
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(80)80717-4
Subject(s) - mechanism (biology) , replication (statistics) , viral replication , virology , chemistry , virus , rna , microbiology and biotechnology , biology , biochemistry , physics , gene , quantum mechanics
RNA-synthesizing preparations containing endogenous viral RNA templates, the so called replication complexes (RC), can be extracted from picomavirusinfected cells (reviewed [ 121). The membrane-free RCs can in turn be separated, by ultracentrifugation, into fractions synthesizing preferentially either singlestranded (ss) or double-stranded (ds) viral RNA species [3-61. What are the differences in the mechanisms responsible for the formation of these two classes of viral RNA. One such difference, which seemed to us worth investigating, is the additional energy demand for the synthesis of ssRNA molecules as compared to the synthesis of dsRNA species. Here we present data indicating that adenylyl @, ~-me~ylene~diphosphonate , an ATP analog possessing a non-hydrolysable linkage between the /3 and y phosphorus atoms, is an acceptable substrate for the synthesis of encephalomyocarditis (EMC) virus-specific dsRNA species but cannot substitute for ATP in the ss~A-synthe~z~g reaction. This result suggests that the synthesis of picomaviral ssRNA species requires, in addition to RNA-polymerase, an ATP-dependent function (cf. [7]).