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Biosynthesis of dolichyl pyrophosphate trisaccharide from synthetic dolichyl pyrophosphate di‐ N ‐acetylchitobiose and GDP‐D[ 14 C]mannose in calf pancreas microsomes
Author(s) -
Herscovics Annette,
Warren Christopher D.,
Bugge Birgitte,
Jeanloz Roger W.
Publication year - 1980
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(80)80314-0
Subject(s) - medical school , carbohydrate chemistry , glycobiology , chemistry , library science , medicine , biochemistry , medical education , computer science , glycoprotein , glycan
The biosynthesis of asparagine-linked oligosaccharides in glycoproteins begins with the transfer of an oligosaccharide from dolichyl pyrophosphate to protein [ 1,2]. The major oligosaccharide thus transferred has a composition of GlcsMangGlcNAcs [3,4], and a scheme for its biosynthesis in vivo has been proposed [5]. However, details of the complete enzymic synthesis of the oligosaccharide moiety remain to be elucidated. We have shown that calf pancreas microsomes are very active in the synthesis of dolichyl pyrophosphate oligosaccharides [6,7]. Using synthetic dolichol derivatives as substrates we have characterized the first two enzymic reactions involved in the synthesis of the oligosaccharide moiety [8,9] :