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Susceptibility of protein synthesis to streptomycin in streptomycin‐producing Streptomyces griseus
Author(s) -
Sugiyama M.,
Kobayashi H.,
Nimi O.,
Nomi R.
Publication year - 1980
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(80)80084-6
Subject(s) - streptomyces griseus , streptomycin , fermentation , library science , engineering , chemistry , food science , streptomyces , biology , computer science , biochemistry , genetics , bacteria , antibiotics
Streptomycin inhibits protein synthesis in the streptomycin-sensitive bacteria by binding to their ribosomes. Since a streptomycin producer must be resistant to streptomycin, it is of interest to know if the protein synthesis of the streptomycin producer is inhibited by its own product. Cella and Vining [l] could not demonstrate the effect of streptomycin on protein synthesis in an in vitro system prepared from Sfreptomyces griseus, because the cell extract contained some factor inhibitory to polyuridylatedependent polyphenylalanine synthesis. Teraoka and Tanaka [2] reported that the binding of dihydrostreptomycin to ribosomes of S. griseus was very low. On the other hand, Piwowarski and Shaw [3] showed that the crude ribosomes of S. bikiniensis (streptomycin producer) bound 0.3 dihydrostreptomycin molecules/ 30 S ribosomal subunit. This paper describes the establishment of an in vitro polyphenylalanine-synthesizing system in S. griseus. Using this system, investigations were carried out on the inhibitory effect of streptomycin on polyphenylalanine synthesis as well as on the binding affinity of streptomycin to the ribosomal subunits.