Premium
Stimulation of oxygen consumption and superoxide anion production in pulmonary macrophages by N ‐formyl methionyl peptides
Author(s) -
Holian Andrij,
Daniele Ronald P.
Publication year - 1979
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(79)81176-x
Subject(s) - superoxide , stimulation , chemistry , medicine , biochemistry , enzyme
A number of metabolic changes occur when phagocytic cells (i.e., polymorphonuclear neutrophils (PMN)) interact with certain soluble and particulate stimuli (reviewed [l-4]). These metabolic changes include an increase in oxygen consumption, stimulation of hexose monophosphate shunt activity, superoxide anion production (O;), hydrogen peroxide prnduction and chemiluminescence. It has been proposed that some of these stimulants act at specific receptor sites on the plasma membrane of the PMN [S-7]. Less information is available concerning the effects of similar metabolic stimuli on the alveolar macrophage [3]. Furthermore, the stoichiometry of the various reactions in the alveolar macrophage, particularly the proportion of the increase in oxygen consumption, that results in the formation of 0; and HzOz has not been established [8,9]. One effective stimulus in the PMN are the soluble N-formyl methionyl peptides [S,lO-121. Attention has been drawn not only to the metabolic but also to the biologic actions of these peptides on phagocytic cells since they are similar in structure to the N-formyl methionyl peptides produced by bacteria. For example, the peptide ,N-formyl methionyl phenylalanine (FMP), has also been shown to induce enzyme release in PMN [ 121 and to stimulate chemotaxis in PMN and guinea pig alveolar macrophages [ 13 1. Here, the effects of FMP on oxygen consumption and superoxide anion production in guinea pig alveolar macrophages are compared to concanavalin A (Con A). Con A is a plant lectin shown to be a reversible soluble