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Immobilized eukaryotic 5.8 S RNA binds escherichia coli and rat liver ribosomal proteins
Author(s) -
Toots I.,
Metspalu A.,
Lind A.,
Saarma M.,
Villems R.
Publication year - 1979
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(79)81113-8
Subject(s) - escherichia coli , ribosomal rna , chemistry , rna , ribosome , ribosomal protein , biochemistry , microbiology and biotechnology , biology , gene
The eukaryotic ribosome contains two lowmolecular weight RNAs, 5 S RNA and 5.8 S RNA (for reviews [ 1,2]). The larger species is specific only for eukaryotic ribosome and, like 5 S RNA, is a component of the large subunit. Earlier we found that the immobilized rat liver 5.8 S RNA forms a complex with 60 S subunit proteins L.5, L6, L7 and L18 [3]. The yeast 5.8 S RNA has been shown to interact also with E. coli ribosomal proteins L18 and L25 141, specific for the prokaryotic 5 S RNA. Here we demonstrate that the immobilized rat liver and bovine 5.8 S RNAs bind rat liver 40 S ribosomal subunit proteins S6, S14 and S23/S24.5.8 S RNAs bind also a set of E. coli 50 S subunit proteins consisting of L2, L17, L19 for the yeast 5.8 S RNA and L2, L17, L19, L20 and L21 for bovine liver 5.8 S RNA. L18 was visible as a minor protein of the yeast 5.8 S RNA-protein complex.