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Increased efficiency in solid‐phase Edman degradation of synthetic peptidyl‐resins using an oxymethylphenylacetamidomethyl‐linkage
Author(s) -
Matsueda Gary R.,
Margolies Michael N.
Publication year - 1979
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(79)80701-2
Subject(s) - edman degradation , medical school , general hospital , library science , citation , chemistry , computer science , medicine , family medicine , biochemistry , medical education , peptide sequence , gene
The solid-phase Edman degradation procedure as proposed by Laursen [ 1] is ideally suited to the evaluation of peptidyl-resin products resulting from Merrifield solid-phase synthesis [2,3] of peptides. Incomplete deprotection and incomplete coupling reactions which occur during synthesis have been detected using quantitative Edman degradation [4,5]. However, extended sequencing experiments were precluded, because the repetitive yield for the Edman procedure averaged 80% [5]. We have also observed repetitive yield values of 80-85% for other peptides synthesized on the same types of benzyl ester resin. In contrast, however, for recent sequence analyses of peptides which were synthesized using a resin with the -oxymethylphenylacetamidomethyl-linkage [63 repetitive yields averaged 95 + 3% (n = 11). In order to study this difference more critically, two model peptides were synthesized using two different peptidylresin linkages. Quantitative sequence analysis of these four peptidyl-resins provided direct comparisons of the influence of: (1)