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Muscarinic receptor‐mediated increase in cyclic GMP level in isolated bovine adrenal medullary cells
Author(s) -
Yanagihara Nobuyuki,
Isosaki Minoru,
Ohuchi Takeshi,
Oka Motoo
Publication year - 1979
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(79)80633-x
Subject(s) - medicine , pharmacology
Adrenal medulla is regarded as a model of neurons containing catecholamine (CA), and is the most suitable tissue for studying the regulation of CA release and synthesis. Recently, some investigations have been carried out on these problems using isolated adrenal medullary cells [ 141. Such preparations are suitable for use in elucidating how stimulation of membrane receptors is coupled with release and synthesis of CA. We have also isolated bovine adrenal medullary cells, which respond to acetylcholine (ACh), nicotine or excess K+ (56 mM), by release of CA and increased synthesis of CA [5]. Here, we examine the effect of cholinergic agents and of excess K’ on the level of cyclic GMP (cGMP) in the isolated cells and found that the cGMP level was increased via stimulation of muscarinic ACh receptors. The increase in cGMP level is considered in relation to the release of CA from the cells. During preparation of this manuscript Schneider et al. [6] reported that the level of cGMP could be increased by ACh at lower concentrations than that required to initiate the release of CA from the isolated ceils.