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Isolation of acth responsive cells from rat adrenal cortex and the determination of the density of male and female cells
Author(s) -
Ungar F.,
Hsiao J.,
Greene J.M.,
Headon D.R.
Publication year - 1979
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(79)80557-8
Subject(s) - medical school , isolation (microbiology) , citation , medicine , library science , chemistry , biology , computer science , medical education , bioinformatics
While density gradient ~e~tr~fugation in gradients of varying shape and density has lung been used in the fractionation of subcellular particles, such procedures have not been widely employed in preparing whole cell populations. This is due largely to the fact that most gradient materials exhibit undesirable properties such as high toxicity, high ionic strength or high viscosity at the ~on&entrations suitable for ceU separations. Metrizamide was developed because of this lack of appropriate gradient materials and has now been used in the separation of a number of different cell types from a variety of tissues [l]. The purj~cation of specific celis from heterogenous populations after tissue dispersion is often essential to the study of celiular biochemistry. In the case of specific endocrine cells such a preparation of isolated cell populations is critical in the determination of mechanisms of hormone action [2,3], The separation and steroidogenic activity of intact adrenal cortical rat cells after density gradient ce~t~fugation in metrizamide is reported here together with an analysis of the density of male and female rat adrenal cortical cells.