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Immunological relatedness of two isozymes of 3‐phosphoglycerate kinase from the mouse
Author(s) -
Pegoraro Bruna,
Ansari Aftab A.,
Lee Chi-Yu,
Erickson Robert P.
Publication year - 1978
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(78)81032-1
Subject(s) - environmental research , library science , basic research , biological sciences , national park , environmental ethics , genetics , biology , microbiology and biotechnology , geography , ecology , philosophy , computer science , environmental planning
Two isozymes of 3-phosphoglycerate kinase (ATP:3-phospho-D-~ycerate 1 -phosphotransferase, EC 2.7.2.3, PGK) are known to be present in mammalian tissues. PGK-I ** is found in all somatic tissues, and its structural locus is X-linked in humans [l] , kangaroos [2] and mice [3] .PGK-2 has been detected only in the sperm and testis of a number of mammals; however, there are exceptions where it has been found in somatic cells (kangaroo, dog, fox [4] ). Alleles determining electrophoretic variation map to chromosome 17 in the mouse [.5] . These two isozymes were purified in our laboratory, and their biochemical properties compared [6]. They were highly similar in their biochenlical properties even though the two loci must have been separated for over 100 million years [7]. To further explore the relatedness of these isozymes, antisera against the two PGK isozymes were produced separately, and their immunological properties were compared. The results of these studies suggest some antigenic relatedness between these two PGK isozymes.