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Regulation of acetylcholinesterase in cultured chick embryo spinal cord neurons
Author(s) -
Walker Kay B.,
Wilson Barry W.
Publication year - 1978
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(78)80810-2
Subject(s) - acetylcholinesterase , spinal cord , embryo , microbiology and biotechnology , aché , anatomy , chemistry , neuroscience , biology , andrology , biochemistry , enzyme , medicine
Primary nerve cell cultures contain acetylcholinesterase (AChE: EC 3 .l .1.7) and other special macromolecules associated with cholinergic transmission [l--4 J . Cultured chick nerve cells, like cultured muscle, have been shown to release AChE into their medium [ 5 $1. The experiments presented here examined the levels of activity and release of AChE in nerve cell cultures after treatment with di-isopropylphospho~uoridate (DFP) and acetylchol~e analogs. The results confirm that dissociated chick embryo spinal cord cells release AC&E and demonstrate that the cells regulate their level of AChE activity in response to chemical treatments.