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On the inhibition of cellulose biosynthesis by coumarin
Author(s) -
Hopp H.Esteban,
Romero Pedro A.,
Lezica Rafael Pont
Publication year - 1978
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(78)80575-4
Subject(s) - humanities , art , political science
1. Introduction It has been reported that coumarin inhibits the incorporation of glucose into cellulose fractions of mung bean [ 11, as well as fiber formation of isolated tobacco leaf protoplasts cultivated in vitro [2]. If the effect of coumarin is specific for the synthesis of cellulose, it could be a very useful compound for the study of cellulose biosynthesis. Particulate prepara- tions of the Chlorophyta Prototheca zopfii catalyzed the following reactions [3]: UDP-Glc t Dol-P + glucolipids + oligosaccharide- linked lipids + glucoprotein + cellulose GDP-Glc We report here that coumarin inhibits the transfer of the oligosaccharide chain from the lipid to the protein acceptor and does not affect the transfer of mannose from lipids to proteins. 2. Materials and methods Prototheca