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Flash polarographic detection of superoxide production as a means of monitoring electron flow between photosystems I and II
Author(s) -
Jursinic Paul A.
Publication year - 1978
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(78)80288-9
Subject(s) - chemistry , photosystem , polarography , photosystem ii , photosynthesis , biochemistry , inorganic chemistry
In isolated chloroplasts exogenous low potential electron acceptors, such as methyl viologen (MV), have been used to study the chloroplast electron transport involving photosystem I. The reduction of acceptors and their consequent autoxidation [ 1 ] can most easily be followed polarographically by the uptake of 02. The reactions which are believed to occur [2,3] when two electrons are transferred from water to MV are as follows: