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A computer analysis of cyanide stimulated oxygen uptake in Chlorella protothecoides
Author(s) -
Clore G.M.,
Chance E.M.
Publication year - 1977
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(77)80819-3
Subject(s) - citation , computer science , library science
Chlorella protothecoides is a unicellular alga which when grown under heterotrophic conditions using a high glucose and low organic nitrogen source has no chlorophyll, little carotenoid and a reduced chloroplast structure [ 1 ]. These properties make it very suitable for respiration studies. Interest in respiration has centred around the presence, characteristics and role of inhibitor stimulated oxygen uptake a property which is widespread among the algae. Despite numerous studies [2-8] the mechanism of inhibitor stimulated respiration is unknown. Grant and Hommersand [9] have investigated the inhibitor responses of whole cells of Chlorella protothecoides quantitatively, and the data thus obtained has been used as the basis of the present study. The present work concerns the elucidation of the mechanism, mathematical symbolism and kinetics of cyanide stimulated oxygen uptake in Chlorella protothecoides by means of non-linear optimization procedures to enable one to test the ability of a given mechanism to reproduce the experimental observations.