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Ecdysterone and acetylcholinesterase activity in cultured Drosophila cells Inducible, non‐inducible and constitutive clones or lines
Author(s) -
Best-Belpomme Martin,
Courgeon Anne-Marie
Publication year - 1977
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(77)80617-0
Subject(s) - ecdysterone , biology , humanities , art , biochemistry , hormone
While examining the morphological effects of the steroid molting hormone ecdysterone (fl-ecdysone) on Drosophila melanogastcr cell line Kc [I ] , various ecdysterone-sensitive and -resistant clones were selected [2] . We demonstrated the presence of ecdysterone saturable binding sites (‘receptors’) in sensitive clones and their absence in resistant clones [3] and showed a timeand ecdysterone concentration-dependent protein induction in sensitive clones [4,51. Recently, Cherbas et al. [6] demonstrated the induction of acetylcholinesterase (AChE, EC 3.1.1.7) activity by ecdysterone in a Drosophila melanogaster X0 and haplo-IV cell line derived from XX Kc cells [I] . Based on the data of Cherbas et al. [6] and to extend the understanding of ecdysterone action, we now report on the existence of four differently responsive cell lines: two are AChE inducible, one is AChE noninducible and one is AChE constit utive.