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Studies of energy‐linked reactions: Oleoyl phosphate‐dependent ATP synthesis (oleoyl phosphokinase) activity of membrane ATPase and soluble ATPases from mitochondria, chloroplasts, chromatophores and Escherichia coli plasma membrane
Author(s) -
Hyams Robert L.,
Carver Mark A.,
Partis Michael D.,
Griffiths David E.
Publication year - 1977
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(77)80608-x
Subject(s) - atpase , chemistry , library science , biochemistry , computer science , enzyme
A cofactor role for lipoic acid and unsaturated fatty acids in oxidative phosphorylation has been demonstrated and net synthesis of ATP catalysed by mitochondrial particles and ATP synthase preparations has been demonstrated utilising oleoylS-lipoate and oleoyl phosphate as substrates [l-5] . Oleoyl phosphate-dependent ATP synthesis by submitochondrial particles and ATP synthase preparations (Complex V) was shown to be insensitive to uncouplers such as FCCP, TTFB and ‘1799’, and to oligomycin and triethyltin, but was sensitive to DCCD and FiATPase inhibitors such as efrapeptin [l] . The oleoyl phosphatedependent ATP synthesis (oleoyl phosphokinase) reaction is thus representative of the terminal reactions of oxidative phosphorylation and provides a new experimental system for study of the capacity for ATP synthesis by a variety of soluble ATPases and coupling factors which have been isolated from bioenergetic membranes [6] , including several soluble preparations with low or latent ATPase activity [7-91. This paper demonstrates that the oleoyl phosphatedependent ATP synthesis (oleoyl phosphokinase) reaction is a characteristic reaction of all bioenergetic membranes including chloroplasts and chromatophores. In addition, it is shown that a soluble ATPase preparation [IO] isolated by chloroform extraction of all bioenergetic membranes, catalyses the oleoyl phosphokinase reaction. Some general features of this reaction catalysed by the soluble ATPase preparation from heart mitochon-