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Biosynthesis of vitamin B 12 Formation of free 5,6‐dimethylbenzimidazole and α‐ribazole from riboflavin by Propionibacterium freudenreichii
Author(s) -
Hörig Joachim,
Renz Paul
Publication year - 1977
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(77)80470-5
Subject(s) - propionibacterium freudenreichii , citation , riboflavin , stereochemistry , chemistry , philosophy , library science , computer science , food science , fermentation
In experiments with radioactive tracers it was shown that the DBI*-moiety of vitamin Blz is formed from riboflavin [l] . Thereby the C-l’ of riboflavin is transformed into C:2 of DBI [2]. These studies were carried out with Propionibacterium shemanii which produces vitamin Blz. In order to isolate the DBI the vitamin Blz was degraded by acid hydrolysis. Until now DBI was never found in free form in vitamin Blz synthesizing bacteria but only as a component of vitamin Blz. In this publication experiments are described demonstrating that cells of P. freudenreichii but not of P. shermanii, grown in the absence of a cobalt salt, transform riboflavin into free DBI, which can be isolated from the incubation medium.