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Differences in membrane structure between suspended and attached mouse neuroblastoma cells
Author(s) -
Erkell Lars J.
Publication year - 1977
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(77)80231-7
Subject(s) - citation , chemistry , microbiology and biotechnology , library science , philosophy , computer science , biology
Fluorescence spectroscopy offers a way to measure biophysical parameters of the membranes of living cells by the use of fluorescent probes [ I,21 . ‘Ihe probe is bound to membrane sites by electrostatic and hydrophobic forces, usually with a substantial increase in fluorescence yield and a shift of the emission maximum toward shorter wavelengths [3,4] . Measurements of this kind can easily be made on a cell suspension with a normal spectrofluorimeter. However, there is evidence that suspension-cultured cells will change their membrane structure upon attachment to a surface [5] . It is therefore possible that results obtained with cell suspensions will not be valid for attached cells. This report describes a method of making fluorescence measurements on attached cells with an ordinary spectrofluorimeter. The cells used, mouse neuroblastoma C 1300 clone 41A3, will grow both in suspension and attached to a surface. Fluorescence measurements using the probes ANS*, DC and NPN were carried out on suspended and attached cells. The results indicate that attached cells have a membrane organization which is different from that of cells in suspension.