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Proprietes cinetiques de la dopamine‐β‐hydroxylase membranaire
Author(s) -
Miras Portugal Maria Teresa,
Jorda Antonio,
Ruiz Angel Santos
Publication year - 1976
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(76)80983-0
Subject(s) - chemistry , solubilization , membrane , substrate (aquarium) , water soluble , enzyme , kinetics , dopamine , biochemistry , chromatography , biology , endocrinology , organic chemistry , ecology , physics , quantum mechanics
We studied membrane bound dopamine‐β‐hydroxylase (DBH) from chromaffin granules, in order to determine whether a biological form of immobilization of the enzyme altered its kinetic properties. The results obtained suggested that DBH either in soluble or solubilized form showed a ping‐pong mechanism whereas the membrane bound DBH did not. Affinity for the substrate and the pH stability were lower in soluble or solubilized form than in membrane bound DBH.