Biosynthesis of purple membrane: control of retinal synthesis by bacterio‐opsin

as follows: 500 ml 230 medium 100 reciprocating movements per min for 5 days at Low purple membrane production is observed under conditions of sufficient aeration: cells were grown in Erlenmeyer flasks containing only 90 ml medium under otherwise in the presence of mM nicotine yielded cells with ml medium) or (90 ml of bacteria-opsin, respectively (‘nicotine [7 ] Basal salt solution contained liter 250 g NaCI, 20 g MgSO‘, . 7Ha 0 and 2 g KCl.