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The use of antibiotics actinomycin D and distamycin A for mapping of phage lambda HindIII fragments
Author(s) -
Braga E.A.,
Nosikov V.V.,
Polyanovsky O.L.
Publication year - 1976
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(76)80733-8
Subject(s) - citation , library science , hindiii , microbiology and biotechnology , physics , biology , genetics , dna , computer science , restriction enzyme
The antibiotics distamycin A and actinomycin D interact with double-stranded DNA forming noncovalently bound complexes. This interaction is rather selective: distamycin forms complexes preferentially with d(A/T) rich regions [ 11 whereas actinomycin binds d(G/C) rich regions [2] of DNA. This property has offered a possibility to use these antibiotics for effective and highly selective protection of the cleavage sites recognized by restriction endonucleases [3,4]. The recognition site of endo R. HindIII [5]