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Convenient procedure for extraction of gonadotropin releasing hormone and thyrotropin‐releasing hormone from hypothalamic tissue
Author(s) -
Koch Y.,
Baram Tallie
Publication year - 1976
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(76)80362-6
Subject(s) - hormone , citation , graduate students , library science , computer science , medicine , medical education
Measurement of tissue concentrations of the hypothalamic hormones gonadotropin-releasing hormone (GnRH) and thyrotropin-releasing hormone (TRH) is essential for studying their physiological roles, since reliable determination of the peripheral serum levels of these hormones has not yet been achieved [1,2]. Only minute amounts of GnRH and TRH are released into the hypothalamo-hypophyseal portal system, and these are greatly diluted in the general circulation. Moreover, these peptides have an extremely short half-life time [3,4] . Thus, at present, study of the fluctuations in the hypothalamic content of GnRH and TRH provides the only means of relating various physiological events to changes in the production and release of these hormones. The established procedures for quantitative extraction of hypothalamic hormones, however, are cumbersome as they involve acidification, readjustment of pH and solvent evaporation. This report presents an alternative, simple procedure for quantitative extraction of GnRH and TRH.