Premium
Validity of PG E 1 radioimmunoassay by using PG E 1 antisera with differential binding parameters
Author(s) -
Maclouf J.,
Andrieu J.M.,
Dray F.
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)81108-2
Subject(s) - citation , philosophy , chemistry , physics , library science , computer science
Prostaglandins (PCs) become immunogenic when their carboxyl groups are coupled by carbodiimide to free NH2 groups of proteins. Thus it is possible to raise PC Fa! antibodies directed mainly against PG Fa [l-3] . But this procedure, when used for the other types of primary prostaglandins, has rarely been successful for production of antibodies with specificity for the homologous hapten. Antibodies obtained in rabbits by immunizing with prostaglandin Er -bovine serum albumin (PG E, -BSA) were directed mainly against PG A, [4] or PG Br [5,6] or did not discriminate between PG AI and PC Br [7] ; antibodies to PG Es-BSA were directed against more PG A2 and PG Bs than PG Es [8,9] . Antibodies to PG A, -BSA cross reacted significantly against PG Er and PG Ez [4] or to a higher extent against PG Br [7] ; and antibodies to PG Bz -BSA cross reacted to a higher extent with PG B1 (unpublished data). Despite the poor specificity of these antibodies, a certain credence has been given to PG Er and PG Al radioimmunoassays [lo] when appropriate chromatography is introduced into the procedure. This report describes the binding parameters of antibodies raised in rabbits and sheep by immunization with the same immunogen PG Er -BSA and presents a comparative study of PG Er levels in human plasma using the rabbit and sheep antisera of the greatest specificity. The effects of greater affinity and specificity of the sheep antisera will be discussed.