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Poids moléculaire d'un récepteur membranaire d'UDP‐glucose
Author(s) -
Berthillier Gisèle,
Frot-Coutaz Jacques,
Got René
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)81104-5
Subject(s) - sephadex , chemistry , size exclusion chromatography , ultracentrifuge , chromatography , sucrose , isoelectric focusing , membrane , microsome , biochemistry , microbiology and biotechnology , biology , enzyme
Rat liver microsomal membranes have been shown to contain an UDP‐glucose binding protein. Its mol. wt was estimated to be 120 000 by gel filtration and by ultracentrifugation in a sucrose gradient. The receptor activity was purified by gel filtration on Sephadex G 200 and analysed by gel‐electrofocusing.