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Evidence for penetrant and non‐penetrant thiol reagents and their use in the location of rat liver mitochondrial D(‐)‐β‐hydroxybutyrate dehydrogenase
Author(s) -
Gaudemer Y.,
Latruffe N.
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)81061-1
Subject(s) - computer science , chemistry
The implication of mitochondrial protein thiols has been postulated in several functions: coupling mechanism, transport, conformations changes, dehydrogenases, etc.. A general review concerning these topics has been published recently [ 11. In order to localize these SH-groups it is necessary to evaluate the penetrability of SH reagents through the inner membrane; this was done by measuring, in the presence of different thiol reagents in isotonic conditions and varying the experimental conditions, the amount of free reduced (GSH) and oxidized (GSSG) glutathione in the matrix compartment. These results put forward two types of reagents: penetrant and non-penetrant thiol reagents. The use of these two types permits to localize the rat liver mitochondrial D(-)-fl-hydroxybutyrate dehydrogenase on the inside part of the inner membrane. Non penetrant thiol reagents do not inhibit the D(-)-/_%hydroxybutyrate oxidation in intact mitochondria but only in sonicated mitochondria whereas penetrant thiol reagents inhibit immediately with intact mitochondria. This location is confirmed by