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Studies on the characteristics of a proton pump in phospholipid vesicles inlayed with purified complex III from beef heart mitochondria
Author(s) -
Guerrieri Ferruccio,
Nelson B.Dean
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)80935-5
Subject(s) - chemistry , arrhenius equation , phospholipid , dept , biochemistry , stereochemistry , activation energy , organic chemistry , membrane
It is well documented that the respiratory chain functions as a proton pump [l] . Two views have been put forward to describe the details of this pumping action. Mitchell’s original hypothesis described the pump as a vectorial transmembrane movement of the hydrogen and electron carriers [ 11. More recently, Papa et al. [2-41 proposed that the redox-proton pump is generated by a linkage between the oxidoreduction of the metal centers of electron carriers and the protonic equilibrium of acidic groups of the apoprotein. Consistent with this suggestion is the demonstration of a proton carrier in the Complex III region of the respiratory chain [3-51 . In this region, no classical hydrogen carriers are known [6-81. More recently, the presence of a proton pump has been demonstrated in phospholipid liposomes containing purified Complex III [9]. In the present paper we -eport a study on some properties of this proton lump, demonstrating its electrogenic nature and pH dependency.