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Biosynthesis of the nucleoside y in yeast tRNA Phe : Incorporation of the 3‐amino‐3‐carboxypropyl‐group from methionine
Author(s) -
Münch H.-J.,
Thiebe R.
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)80900-8
Subject(s) - methionine , transfer rna , yeast , biosynthesis , amino acid , chemistry , biochemistry , stereochemistry , rna , gene
The nucleoside Y in yeast tRNAflle [ 1,2] and in the tRNAPhe of several other organisms [3-61 has one of the most complex structures of all modified nucleosides known. The biosynthesis of this nucleoside is not evident from its structure; we only know that it is derived from guanosine [7,8]. Nishimura et al. [9] found that the 3-amino-3carboxypropyl-group can be transferred from S-adenosylmethionine onto uridine to form the odd nucleoside X in E. coli tRNA. The hypothesis for the present study was a similar pathway in the synthesis of Y. A part of the side chain, as represented by the large characters in fig. 1, might be derived from methionine. A yeast strain auxotroph for methionine was grown on radioactive methionine with the marker in the carboxyl group. This radioactivity was shown to be incorporated into the nucleoside Y, without isotopic dilution. Practically no radioactivity was found in the