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The formation of Helix pomatia methaemocyanin accelerated by azide and fluoride
Author(s) -
Witters Raphael,
Lontie René
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)80758-7
Subject(s) - helix pomatia , citation , computer science , library science , chemistry , biology , ecology , snail
Haemocyanin, the oxygen-carrying pigment from the haemolymph of the mollusc Helix pornaria, shows two copper-oxygen absorption bands with a maximum at 346 and at 580 nm. These bands decrease slowly on storage [ I] . They reach about half their original value in one year and can be regenerated with hydrogen peroxide [2) and with hydrogen suiphide [3]. A considerable decrease of the copper bands after a few weeks, observed in the separation of cyand phaemocyanin in the presence of 1 M NaCl at pH 5.7, incited us to investigate the influence of halogenides. Similarly the abnormal bleaching of the colour of fresh haemocyanin by the addition of 3 mM sodium azide as a preservative led us to try the action of azide (C. Gielens, unpublished observation).