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Konformationsänderungen vor und nach DNA‐synthese verstärken zellgenetisch letale strahlenschäden
Author(s) -
Hagemann Gerd,
Mellmann Jürgen
Publication year - 1975
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(75)80755-1
Subject(s) - chinese hamster ovary cell , chemistry , mitosis , microbiology and biotechnology , dna , irradiation , radiation sensitivity , biophysics , biology , biochemistry , physics , nuclear physics , receptor
Chinese hamster ovary fibroblasts were synchronized by mitotic selection and showed cytogenetic lethally acting radiation damages, which depended on age at irradiation time. The development of cell cultures was followed by counting of the killed cells in the 3rd daughter generation of the irradiated ones and by evaluation of the colony size spectra 73.5 h after innoculation. At a dose of 300 rads two narrow but high peaks of radiation sensitivity were induced, which appeared at the beginning and at the end of the S‐phase of the cell cycle and therefore are reduced to conformational changes of the DNA. Consequently the DNA‐superstructures linking pieces of the nucleoprotein‐fibre during fastening to, and detaching from, the nuclear membrane are assumed to be the loci of the increased radiation sensitivity.