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Phosphate‐dependent effects of palmityl‐CoA and stearyl‐CoA on phosphate‐activated pig brain and pig kidney glutaminase
Author(s) -
Kvamme Elling,
Torgner Ingeborg Aasland
Publication year - 1974
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(74)81021-5
Subject(s) - neurochemical , phosphate , psychiatry , psychology , biology , biochemistry , neuroscience
Phosphate-activated glutaminase (EC 3.5.1.2) purified from pig kidney [l] and brain [2] , has been shown to be activated by low concentrations of Bromothymol Blue (< 0.05 mM) [ 1,3] , and inhibited by higher concentrations. The dye has, thus, a dual and unique effect on the enzyme. Furthermore, the activation is potentiated by phosphate, and phosphate also protects against inhibition by the dye. In a search for physiological compounds with similar properties to Bromothymol Blue, acetyl-CoA has been found to be an activator of glutaminase with I(, about 0.2 mM [4]. As the activation by acetyl-CoA is diminished by phosphate and other anions such as citrate, the mode of action is different from that of Bromothymol Blue. We show here that palmityl-CoA and stearyl-CoA in principle have similar properties to Bromothymol Blue.