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Étude comparative de la biosynthèse des phosphatidylcholines dans les neurones et les cellules gliales du cerveau de poulet
Author(s) -
Freysz L.,
Mandel P.
Publication year - 1974
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(74)80905-1
Subject(s) - phosphocholine , choline , chemistry , transferase , biochemistry , enzyme , diglyceride , microbiology and biotechnology , biology , phosphatidylcholine , phospholipid , membrane
The activity of CDP‐choline 1,2‐diglyceride phosphocholine transferase was studied in chick brain neurons and glial cells. The specific activity was 2.7‐fold higher in the neurons than in the glial cells. Both cell types had an optimum activity at pH 8.0.Mg 2+ and Mn 2+ activated the enzyme, while Ca 2+ was inhibitory. CDP‐ethanolamine and CMP were competitive inhibitors. The apparent K M of the enzyme for the CDP‐choline was found to be 0.39 × 10 −4 M in the neurones and 0.17 × 10 −4 M in the glial cells. This suggests the existence of isoenzymes for the CDP‐choline 1,2‐diglyceride phosphocholine transferase in chick brain.