Premium
Purification of HL‐A antigens from the urine of diseased and healthy individuals
Author(s) -
Bernier I.,
Dautigny A.,
Colombani J.,
Jollès P.
Publication year - 1974
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(74)80868-9
Subject(s) - biochemistry , transplantation , medicine , library science , computer science
The HL-A antigens used for most of the biochemical studies in the past have been solubilized from cellular materials. However in such cases the recovery of purified active molecules is always low, no matter which solubilization technique is used. Accordingly. a richer and/or more abundant source of HL-A antigens which can easily be purified had to be sought. Two recent publications have reported such sources: Billing and Terasaki [l] showed that large quantities of HL-A antigens are present in human serum, and Robert et al. [2] were able to demonstrate the presence of HL-A antigens in the urine of patients suffering from tubular proteinuria. Since urine is abundant and easy to collect, we have partially purified HL-A antigens from that of diseased and of normal individuals.