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Parameters influencing the purification of calf uterus estrogen ‘receptor’ by affinity chromatography
Author(s) -
Truong Hélène,
Baulieu Etienne-Emile
Publication year - 1974
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(74)80397-2
Subject(s) - uterus , affinity chromatography , estrogen receptor , estrogen , chemistry , chromatography , receptor , endocrinology , medicine , biology , biochemistry , enzyme , cancer , breast cancer
The partial purification of the e&radio1 ‘receptor’ from calf uterine cytosol, using new 7ar-estradiol derivatives for affinity chromatography, has been describ ed in a preliminary report [l] . Early attempts were made to purify a ‘4 S’ form of the ‘receptor’ from calcium treated cytosol [l-4] . However this form of the receptor was only obtained from fresh uteri, since all efforts to prepare it from frozen uteri resulted in very low yields [5]. Thus, it was difficult to undertake a program of large scale purification which a priori requires the storage of frozen uteri. This problem has been partially resolved by using a ‘4 S’ form of the ‘receptor’, which can be obtained, from both fresh and frozen uteri, by trypsin treatment of cytosol [6]. This paper will present some systematic studies which were undertaken on the parameters affecting the purification of the ‘receptor’ by affinity chromatography.