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Prevention of hemolysis by bivalent metal ions during virus‐induced fusion of erythrocytes with ehrlich ascites tumor cells
Author(s) -
Zakai Nehama,
Loyter Abraham,
Kulya Richard G.
Publication year - 1974
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(74)80256-5
Subject(s) - hebrew , chemistry , citation , philosophy , classics , library science , computer science , history
Chicken erythrocytes have recently been fused to form homopolykaryons with Sendai virus in the presence of bivalent cations [ 1,2] or with the aid of lysolecithin [3]. Human erythrocytes could also be fused with each other under similar conditions [4,5] . When adult chicken erythrocytes were fused with other cells only the nuclei of the erythrocyte were incorporated into the heterokaryon while the cytoplasm was lost by lysis [6] . It seemed of considerable potential interest to form heterokaryons of erythrocytes with other cells without hemolysis since this would make it possible to study the effects of erythrocyte cytoplasm on the activity of the nucleus and cytoplasm of other cell types. The aim of the present investigation was to find conditions for fusing erythrocytes with other cells without the loss of erythrocyte cytoplasm. This paper shows that the techniques developed for the formation of homopolykaryons [2,4] may be applied with slight modification to the fusion of chicken and human erythrocytes with other cells without loss of erythrocyte cytoplasm.