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Ribonuclease‐sensitive DNA polymerase activity associated with particles distinct from A type and C type viral particles in murine myeloma tumor cells
Author(s) -
Penit C.,
Paraf A.,
Rougeon F.,
Chapeville F.
Publication year - 1974
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(74)80111-0
Subject(s) - biology , microbiology and biotechnology
Murine myeloma tumor cells in culture produce intracisternal, A-type, virus-like particles, as well as C-type particles [l-3]. Wilson and Kuff [4] have shown that A-type particles contain an enzymatic activity responsible for the polymerization of dTMP in the presence of the synthetic hybrid poly rA*oligo dT, but they did not find any endo~~no~s ~bonuclea~sensitive DNA polymerase activity similar to that of oncornaviruses [S-S], In this paper we report studies on two DNA pdymerase activities present in particulate extracts of mym the other is responsible for the poly rA*oligo dT-directed dTMP polymerization. In order to characte~ze the particles bearing the endogenous activity and to compare them with virus-like particles, fractionation experiments of the MF cell extracts were carried out. In addition, the two DNA polymerase activities were studied in extracts from MF cell-induced solid mice tumors, which produce mostly A-type particles, and in extracts of an epithelioid cell variant (referred to as ME cells, [9, 101) which produces neither Anor Cstype particles.