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A novel system for DNA synthesis in isolated nuclei
Author(s) -
Lazarus L.H.
Publication year - 1973
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(73)80602-7
Subject(s) - hebrew , citation , philosophy , physics , microbiology and biotechnology , chemistry , library science , computer science , biology , linguistics
Isolated, intact mammalian nuclei have been employed in the study of DNA synthesis from normal [l-6] and viral infected cells [7-91 . Nuclear suspensions appear to be readily permeable to both macromolecules [ 1,6] and nucleoside triphosphates with a requirement for ATP in DNA synthesis [2-4,6,7] , analogous in this respect, to toluenized bacteria [lo]. In this communication, a novel system is described for the assay of DNA synthesis in nuclei based on the cellophane disc method of Schaller et al. [ 1 I] . The activity, which reflects the physiological state of the cells, is linear for at least 2 hr in the presence of all four ribonucleoside triphosphates.