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Translation of oncogenic viral RNA and eukaryotic messenger RNA in the E. coli cell‐free system
Author(s) -
Gielkens A.L.J.,
Salden M.H.L,
Bloemendal H.
Publication year - 1972
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(72)80747-6
Subject(s) - nijmegen breakage syndrome , biology , molecular cell biology , rna processing , genetics , microbiology and biotechnology , rna , gene , dna , dna damage , ataxia telangiectasia
Recently several studies have been published which describe the faithful translation of animal messenger and viral-RNA in eukaryotic cross systems [l-l 21. Although stimulation of amino acid incorporation by viral RNA or eukaryotic messengers in bacterial cell-free systems has been reported, reliable translation has only been described incidentally [ 13191. We compared the translation of eukaryoticand oncogenic viral-RNA’s in the cell-free system of E. coli with that of the reticulocyte lysate. Our results provide evidence that in the bacterial system the viral RNA’s are translated into several distinct polypeptides, whereas no indication for the translation of eukaryotic messengers could be obtained. Moreover, addition of the viral RNA’s to the mammalian systems described, appeared not to result in translation.