Premium
3 H‐labelling of a synthetic decapeptide having LH and FSH releasing activity (LH‐RH/FSH‐RH)
Author(s) -
Marche P.,
Morgat J.L.,
Fromageot P.,
Kerdelhue B.,
Jutisz M.
Publication year - 1972
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(72)80547-7
Subject(s) - chemistry
A decapeptide capable to stimulate the release of LH and FSH from pituitary has been recently isolated from hypothalami of porcine origin and its amino acid sequence has been established as (Pyro)Glu-HisTrp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH, [ 1,2]. An identical decapeptide has been found in sheep hypothalami [ 3,4] . Several laboratories described syntheses of LH-RH/FSH-RH either by the solid phase technique [5, 81 or by the classical approach [9, lo] . Previous studies in our laboratory have permitted the tritiation of several peptidic hormones with subsequent retention of their biological activities. The method used, described by Morgat et al. [ 11, 121, consists in a catalytic hydrogenolysis of iodinated tyrosyl residue(s). Recently, by a similar procedure, Pradelles et al. [ 131 and Menez et al. [ 141 have obtained [3H]TRH and Nuja nigricollis [3H]a-neurotoxin, respectively, in which the histidyl side chain carried the label. The present report describes the tritium labelling of LH-RH/FSH-RH. The product had a specific radio. activity of 75 Ci/mmole, it was biologically active on the release of both LH and FSH, and not distinct in this respect from the unlabelled decapeptide. 2. Experimental